Biochemical analysis of the 20 S proteasome of Trypanosoma brucei.

alpha5 subunit in Trypanosoma brucei proteasome can self-assemble to form a cylinder of four stacked heptamer rings.

The proteasomes have a central role in catalysing protein degradation among both prokaryotes and eukaryotes. The 20 S proteasome constitutes their catalytic core. In studying the structure of Trypanosoma brucei 20 S proteasomes, we isolated by two-dimensional (2D) gel electrophoresis a 27 kDa subunit protein with an estimated pI of 4.7 and subjected it to mass spectrometric analysis. A tryptic ...

[ 25 ] Purification and Analysis of Recombinant 11 S Activators of the 20 S Proteasome : Trypanosoma brucei PA 26 and Human PA 28 , PA 28 , and PA 28

Proteasomes perform the bulk of nonlysosomal degradation of aberrant, damaged, misfolded, and naturally short‐lived regulatory proteins in eukaryotic cells. They are 700‐kDa assemblies whose hollow architecture sequesters the proteolytic sites inside a central chamber, thereby ensuring that the activity of isolated proteasomes is repressed. In vivo, proteasomes are activated by protein complexe...

Structural and biochemical analyses of the eukaryotic HslV from Trypanosoma brucei*

In many bacteria, heat shock locus V (HslV) functions as a protease, which is activated by heat shock locus U (HslU). The primary sequence and structure of HslV are well conserved with those of the β-subunit of the 20S proteasome core particle in eukaryotes. To date, the HslVU complex has only been characterized in the prokaryotic system. Recently, however, the coexistence of a 20S proteasome w...

Efficacy of repeated doses of diminazene aceturate (Dinazene®) in the treatment of experimental Trypanosoma brucei infection of Albino rats

The efficacy of repeated doses of Dinazene® in Albino rats experimentally infected with Trypanosoma brucei (Gboko strain) was investigated. A total of 30 adult female Albino rats weighing 130-190 g were used for the study. They were assigned to six groups (groups A-F) of five rats each. Groups A-D were infected intraperitoneally with 1.0 × 106 trypanosomes in 400 μL of PBS diluted blood while g...

Multiplex analysis of RNA interference defects in Trypanosoma brucei.